Composite

Part:BBa_K3064024:Design

Designed by: Hanxiao Feng   Group: iGEM19_NUDT_CHINA   (2019-09-28)


mmuGlucagon-GGGGS-mmulgG-Fc-P2A-HA-mmuTrim21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 800
    Illegal BamHI site found at 1503
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 901
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 125


Design Notes

We have taken the order of each part in the composite part into consideration.We also have to take the stability and capability to express into account while designing.When we choose the linker part we care about if there is any potential effect on other basic parts as well as the whole part's stability and we finally choose GGGGS and P2A as well as HA.


Source

This part is assembled from BBa_K3064021,BBa_K3064023,BBa_K3064009,BBa_K2653003,BBa_K2653004 and BBa_K3064010.

References